Inkubation

Incubation

Establishing a controlled environment that imitates physiological conditions is crucial for promoting cellular growth. It is therefore essential to provide a consistent temperature, pH levels, and an energy supply. The desired environmental conditions can be created through the use of reliable equipment and an appropriate cell medium.

Header Grafik

Incubation of cells

CO₂ incubators:

The environment that allows for maximum cell propagation is achieved primarily through the incubator, the cell culture medium and its supplements. Extended exposure to the outside environment can elevate the risk of cell death, making CO2 incubators essential for the storage and propagation of in vitro cultivated cells. For most mammalian cells, incubation requirements include 37°C temperature, 95% relative humidity, and 5% CO2, all of which can be maintained by specialized CO2 incubators, in particular the INCUBATOR, which we highly recommend.

Culture vessels:

Cells can be cultivated and stored in different culture vessels. Particularly apt for cell culture, are cell culture flasks with filter caps that allow for the exchange of gaseous substances but protect the culture from contaminating agents. Alternatively, we offer classic culture flasks with vent caps or multiple well plates (6-wells, 12-wells or 96-wells).

Light microscopes:

Regularly monitoring the health and growth of the cultured cells is good practice. Trough frequent checks it is possible to detect contaminations, verify cell morphology and attachment, and to assess the confluence. Light microscopes offer a convenient means of observing and recording cell behavior over time.

Helpful articles for incubation