Auftauen und Aussaat

Thawing
and sowing

Freezing and thawing can be quite challenging for a cell culture. To ensure high viability of cryopreserved cells, it is essential to perform the thawing process promptly and treat the cells with great caution. We are pleased to provide a variety of specialized equipment that can assist you in the delicate handling of cryopreserved cell lines.

Thawing and seeding frozen cells

Collect your vial from the liquid nitrogen container, making sure to wear adequate protective gear (lab coat, cryo-Gloves and protective glasses). Be sure to handle everything with care as in rare occasions the vial may explode do to the rapid expansion of trapped liquid nitrogen.
Immediately immerge the vial in a 37˚C warm water bath. As soon as only a few ice crystals are left, disinfect the vial with 70 % Ethanol and transfer it to a laminar flow hood.
To minimize osmotic stress acting on your cells, add 1 mL of the pre-warmed (37˚C) and appropriate cell culture medium in a drop-wise manner (Pipette boy, serological pipettes). Transfer the solution containing your cells (again dropwise) to the remaining volume of your chosen cell medium.
Centrifuge the cells for 3 minutes at 300 g and then carefully aspirate the supernatant to remove any remaining cryo-preservatives, being careful not to disturb the cell pellet. Lastly, resuspend the cells in pre-warmed culture medium and transfer them to the desired culture vessel.
Label the container (Cell line, date and passage). Place the culture vessel in the incubator. If working with adherent cells check after 24 hours for cell attachment.