Sartorius® Vivacon 500, 100,000 MWCO Hydrosart, 25 pcs.

Vivacon 500 is optimally tuned for concentration and buffer exchange of genomic DNA and peptide samples of up to 500 µl starting volume. They are also the product of choice for peptide fractionation in this volume range. To achieve optimal results with highly diluted samples, e.g. forensic sample material, Vivacon 500 is equipped with the patented Hydrosart® regenerated cellulose membrane. Selected cut offs are also available sterile and DNA free* (PCR grade units). For this purpose, the units have been gassed with a dual cycle ethylene oxide (ETO) and are thus sterile up to a safety assurance of 106 and frei of detectable human genomic DNA. This has been verified by a 55 cycle qPCR assay with a detection limit of < 2pg.High recoveries and excellent reproducibility are complemented by the user-friendly scaling of each unit and printed MWCO. Vivacon 500 has a horizontally oriented membrane that is ideally suited for both DNA fractionation applications (primer removal) and peptide fractionation applications (Filter Aided Sample Preparation, FASP1). The possibility of reverse centrifugation of the sample unit promises complete recovery of the concentrate in a second step and is particularly important when working with very dilute samples.1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62. concentration of up to 0.5 ml of genomic DNA or peptide samples Low binding material Possibility for reverse centrifugation MWCO printed Complete and highly reproducible recovery of the concentrate Selected cut-offs are also available sterile and DNA-free*, e.g. for applications in forensic science.E.g. for applications in forensics * qPCR assay over 55 cycles with an assured detection limit of < 2 pg Performance characteristics for DNA Start volume 0.5 ml, sample concentration 50 ng/ml. Sample gre (bp) Time required to concentrate up to 30x [min] at 20C Concentrate recovery % g-force (xg) 2,000 MWCO 10 60 min 93 % 7,500 10,000 MWCO 30 25 min 94 % 7,500 30,000 MWCO 50 18 min 88 % 5,000 50,000 MWCO 300 18 min 91 % 5.000 100,000 MWCO 600 10 min. 87 % 3,000 125,000 MWCO 650 12 min. 85 % 2,000 125,000 MWCO 900 9 min. 94 % 3,000 Performance characteristics for proteins Start volume 0.5 ml, sample concentration and concentration of proteins as listed in the table. Sample Time required to concentrate up to 30x [min.] at 20C Concentrate- Recovery % g-force (xg) 2,000 MWCO 0.25 mg/ml cytochrome c 30 min. 95 % 14,000 10,000 MWCO 0.25 mg/ml cytochrome c 15 min. 92 % 14,000 30,000 MWCO 1.0 mg/ml BSA 10 min. 95 % 14,000 50,000 MWCO 1.0 mg/ml BSA 10 min. 92 % 14,000 100,000 MWCO 1.0 mg/ml IgG (bovine) 11 min. 90 % 8,000 125,000 MWCO 1.0 mg/ml IgG (bovine) 10 min. 81 % 8,000