Sartorius® Complete accessories for Vivaflow 50R|200 system with 115 V pump

Vivaflow 200 is a ready-to-use, reusable tangential flow filtration unit in an acrylic housing, which allows cleaning at high pH values and thus reuse 4-5 times. The membrane area in the unit is 200 cm².One package size includes one unit and all accessories for use with a laboratory peristaltic pump and a size 16 pump head.Two units can be used in parallel for up to 5 L samples to accelerate concentration. Vivaflow 200 is ideally suited for applications such as concentration of cell culture supernatants, viruses and also water concentration e.g. from water samples or from fish breeding tanks. This cost-effective unit is easier to operate than any other laboratory filtration unit of its size, and is also reliable and flexible to use. One unit typically concentrates samples of one liter volume by a factor of 50 in less than 30 min. Alternatively, two Vivaflow 200 units can be used to concentrate 5 l samples in under 75 min. With a simple rinse of the unit, almost complete recovery of the concentrated sample is achieved. The Vivaflow 200 unit can be easily regenerated and reused several times. One packaging unit includes all accessories for use, except a pump. A sealed 500 ml diafiltration cup can be added to the system for easy and fast buffer exchange. Vivaflow 200 complete system Required equipment A Vivaflow 200 packaging unit includes tubing, pressure gauge, flow restrictor and pump tubing. Only one peristaltic pump with a pump head suitable for 6.4 mm OD tubing (size 16) is required for use. Parallel operation of 2 Vivaflow 200 units Two Vivaflow 200 units can be conveniently connected to each other using the ready-to-use Y-connector. The Y-connector is supplied pre-configured with tubing and luer locks. Note that size 16 tubing connects the 2 Vivaflow 200 units together, while size 15 tubing goes into the pump. Thus, you need a size 15 pump head if you want to work with two Vivaflow 200 units in parallel. High flow rate and recovery Clear housing for optimal process tracking Single units are ready to use Two units are easy to configure Reusable product Performance features Used time to concentrate up to 20x [min.] to concentrate at 3 bar inlet pressure, 20C starting volume average flow recovery % 1 liter ml/min Direct 25 ml Splbad Lysozyme 0.25 mg/ml (14,000 MW) 2.000 MWCO Hydrosart 160 6 97 % > 99 % 3.000 MWCO PES 180 5 97 % > 99 % BSA 1.0 mg/ml (66.000 MW) 5.000 MWCO PES 29 33 98 % > 99 % 5.000 MWCO Hydrosart 70 14 98 % > 99 % 10.000 MWCO PES 23 41 96 % > 99 % 10.000 MWCO Hydrosart 35 27 98 % > 99 % 30,000 MWCO PES 25 38 96 % 99 % 30,000 MWCO Hydrosart 20 48 96 % > 99 % 50,000 MWCO PES 22 43 96 % 98 % Globulins 1.0 mg/ml (average 160,000 MW) 100,000 MWCO PES 54 18 96 % 99 % 100,000 MWCO RC 45 21 96 % 99 % Yeast 1.0 mg/ml (S. Cerevisiae) 0.2 m PES 11 86 92 % 98 % Verdnnte solution concentration, start volume 1 liter at 3 bar, 10,000 MWCO PES BSA 0.001 mg/ml 18 52 90 % 98 % BSA 0.01 mg/ml 20 47 92 % 98 % BSA 0.1 mg/ml 21 45 94 % 99 % Start volume 5 liters (two VF200 in parallel at 3 bar) 10,000 MWCO PES BSA 1.0 mg/ml (66,000 MW) 67 70 97 % > 99 %