SERVA SERVALight Vega CL HRP WB Substrate Kit 500 ml

Highly sensitive, enhanced chemiluminescent substrate for the detection of immobilized proteins (Western blot) or nucleic acids (Southern and Northern blot) either directly labeled with horseradish peroxidase (HRP) or indirectly labeled with HRP-labeled antibodies/streptavidin.
Substrate is quickly prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a 1:1 ratio.

• High sensitivity, detection limit in the mid-picogram range
• Directly compatible with protocols of standard ECL-WB substrates from other suppliers
• Low background, high signal/background ratio
• Working solution is stable for at least 5 days, ensuring reproducible results and reducing waste
• Primary antibody dilution 1:100 - 1:5000
• Secondary antibody dilution 1:1000 - 1:15 000
• Detection can be done preferably with a CCD image documentation device or film.