SERVA SERVALight Polaris CL HRP WB Substrate Kit 250 ml

Highly sensitive, enhanced chemiluminescent substrate for the detection of immobilized proteins (Western blot) or nucleic acids (Southern and Northern blot) either directly labeled with horseradish peroxidase (HRP) or indirectly labeled with HRP-labeled antibodies/streptavidin.
Substrate is quickly prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a 1:1 ratio.
- High sensitivity, detection limit in the lower picogram range
- Long light emission over 6 hours
- Primary antibody dilution 1:1000 - 1:5000
- Secondary antibody dilution 1:20 000 - 1:100 000
- Detection can be done with film or a CCD imaging device