SERVA SERVALight Eos CL HRP WB Substrate Kit 250 ml

Highly sensitive, amplified chemiluminescent substrate for the detection of immobilized proteins (Western blot) or nucleic acids (Southern and Northern blot) either directly labeled with horseradish peroxidase (HRP) or indirectly labeled with HRP-labeled antibodies/streptavidin.
Substrate is quickly prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a 1:1 ratio.
- Very high sensitivity, detection limit in the mid-femtogram range
- Very long and stable light emission over 12 hours
- Primary antibody dilution 1:1000 - 1:15 000
- Secondary antibody dilution 1:25 000 - 1:150 000
- Detection can be done with film or a CCD image documentation device